James Joyces Portrayal of Dublin as a Paralyzed Country Term Paper

James Joyces Portrayal of Dublin as a Paralyzed Country - Term Paper Example As Parrinder likewise states, â€Å"Dubliners double-crosses less deceptions about Ireland and Joyce’s way is thoroughly segregated and fair-minded. He is a naturalist to the degree that he permits the loss of motion of the Dublin society to double-cross itself instead of examining it or decrying it openly† (43-44). In addition Joyce himself guarantees that, â€Å"My expectation was to compose a section of the ethical history of my nation and I picked Dublin for the scene since that city appeared to me the focal point of paralysis† (Leonard 320). Such a projection of loss of motion is additionally seen in the narratives, The Sisters, Eveline and The Dead. 1.1. The Sisters The story is portrayed in first individual portrayal delineating a story of a Priest’s passing named Father Flynn. The storyteller was a companion of the cleric who delineates the story of the occurrences that happen after his passing and battles to gather pieces of information about the transgressions of the Father for which the Father had needed absolution. 1.2. Eveline repeats an account of a young lady. Since she was conceived she had experienced a troubled and an intense life. She had consistently wanted for a departure from her life in Dublin despite the fact that whenever destiny presents her with that brilliant possibility she doesn't have the mental fortitude to benefit it since she can't disregard her different duties and leave her past for another future. 1.3. The Dead The Dead is one of the artful culminations of James Joyce in light of the fact that in this story the creator has fused the concentrate of the considerable number of topics that Joyce is seen to be creating in his other short stories. It has an assortment of characters from different age bunches who have accumulated for a musicale. Anyway the essential focal point of the story is on Gabriel and the way in which he manages the individuals around him. 2. Projection of Paralysis In the story T he Sisters it is expressed, â€Å"I delicately said the word paralysis†¦it sounded to me like the name of some pernicious and evil being† (2) this remark can be deciphered as Joyce’s style of building up his abhorrence for the state of his comrades in the principal short story of his assortment. The major topical worries that mirror this loss of motion incorporate religion and analysis of the Church, seclusion, rot and idealism. C. A. Malcolm and D. Malcolm express that, â€Å"through his delegate characters and deliberately created condition, Joyce enlightens the powers that decide, yet so that we can consider those to be as detaining, official, paralyzing† (166). 2.1. Religion is a repetitive subject in the greater part of Joyce’s short stories as he censures the smothering injuries of catholic accepts and subsequently he is seen to condemn and scrutinizing the blessed holy observance of the Catholic Church. Anyway the characters represent the immo bile condition of his kinsmen who never scrutinized the activities of Church and died their lives as per the smothering and keeping limits set by the Catholic Church. The way wherein the character of the minister is delineated in the story and the trace of his transgressions repeats that in spite of the fact that congregation is blessed foundation yet even the devout individuals submit sins albeit nobody could ever voice out such a sentiment. As the storyteller says that he considered the to be in his fantasy as referenced in the tex

Overview Of The Communication Process Information Technology Essay

Outline Of The Communication Process Information Technology Essay Correspondence is an educated expertise. We should figure out how to talk too and correspondence viably even a large portion of us are brought into the world with the physical capacity to talk. There are different approaches to understanding the verbal and nonverbal implications through our talking, learning and capacity ability. We become familiar with the essential correspondence ability by watching others and demonstrating our practices dependent on what we see. We additionally showed some correspondence expertise through training, by rehearsing aptitude and having them assessed. In basic terms, correspondence can be characterized as an activity. Correspondence not just in composed structure or oral, even a delicate touch or a realizing look can likewise impart a message boisterous and clear, as can an irate slap or a hard push. Take a model: babies impart fundamental needs through crying. It does exclude language and dont must be vocalized. It is only a basic transmit data starting with one individual then onto the next individual. It must have a message that being moved from sender to collector in each occurrence of correspondence. The sender and beneficiary must have a few words, signs o flags in the same manner as one another to ensure for the correspondence to be achievement with the goal that message can simple to convey and comprehended. Correspondence is likewise a two-route connection between to gatherings to transmit data and common comprehension between themselves. The exchange of data starting with one gathering then onto the next is best when a conversation accessible, it can makes the correspondence simpler on the grounds that the collector can pose inquiries and get answer to explain the back rub. 1.1 Communication Process The correspondence procedure is the guide frog acknowledging compelling correspondence. Viable correspondence prompts understanding, it is additionally chances to turn out to be increasingly gainful if that follow the correspondence procedure. Sender and the beneficiary occur in the sharing through the correspondence procedure. Correspondence process is a lot of steps that will attempted in an association while each time formal correspondence. It is assists with guaranteeing that your partners are kept routinely educated. The correspondence procedure is made by four key of parts. Those segments incorporate encoding, channel, disentangling, and input. Sender and the recipient are additionally the two different factors all the while. The correspondence procedure starts by the sender and closures with the collector. Clamor in the correspondence procedure is any sort of interference which can upset the correspondence. 1.1.1 Sender (encoder) Sender is the manager who discharge the message started. The sender must detail to encoding so as to pass on importance. You likewise should be positive about the data that you convey is helpful and exact. An uplifting mentality of the communicators and the significant images select by the communicators are the factor that will decide how compelling the communicator will be. 1.1.2 Encoding Encoding is the procedure that moving the data you need, and effectively decoded at the opposite end with the type of imparted. Capacity of pass on data obviously and essentially, take out and envision wellsprings of disarray will profoundly influenced whether achievement or not of your encoding. Realizing your crowd is a significant key piece of encoding, bring about conveying messages will be misjudged if your are inability to comprehend who you are speaking with. 1.1.3 Channel (mode of transmit) Channel implies use to pass on the message with non-verbal and verbal cooperation. The sender utilizes a channel to start transmit their message. The viability of the various kinds of channels vacillates depend the trait of the correspondence. Most stations are letters, updates, messages, reports, meeting, phone and videoconferencing. There are various qualities and shortcomings of various channel. For instance, oral correspondence channel become increasingly powerful when prompt criticism is essential, since it tends to be cleared up on the spot of a vulnerabilities. With the goal that select the fitting direct will significantly aid the adequacy of recipients understandings. 1.1.4 Decoding Translating is acted in beneficiary side in the correspondence procedure. Disentangling is at one time the message is gotten and inspected, the improvement is sent to the cerebrum for deciphering, so as to allot some kind of importance to it. The collector will effectively deciphers the senders message is the correspondence happens fruitful. 1.1.5 Receiver (decoder) Beneficiary is the individual who essentially getting the message. Correspondence will just fruitful when the response of the recipient is what the communicator expected. All the translation by the beneficiary is affected by their insight, discernment, perspectives, encounters, aptitude, and culture. It is really like the senders relationship with recoding. 1.1.5 Feedback Input is the answer we get to the message sent. It might a solicitation for additional explanation, a reactions oral or composed criticism, and unwanted choice or an itemized report. The sender can't affirm whether the recipient has deciphered the message accurately or not without input. It likewise gives a chance to the sender to maintain a strategic distance from an inappropriate activity and to explain a misconstrued message. 1.1.6 Noise Any things will meddle the comprehension of correspondence additionally called as clamor. In this occasion, the message was not appropriately built and henceforth the secretary didn't comprehend it as planned by the sender. In any way, commotion is any upgrade that will be meddles with the communicators capacity to accomplish the comprehension. The approaches to guarantee the target group to get right message A decent correspondence ability can guarantee the target group to get the correct message and it can decrease the misconstrued on message recipient. There have some compelling approaches to guarantee the planned to get a correct message: Culmination: The message must be finished to bring the alluring outcome. You should comprehend what is the peruser needs or needs and you should likewise give all the fundamental data to address the whole inquiry. You can likewise give some additional data when alluring. Complete and Conciseness: Eliminate the tedious articulation, must maintain a strategic distance from unnecessary channel words and expression. Inside the correspondence, you should incorporate the main significant materials, dun need incorporate any un-important data to befuddle the message beneficiary. Solidness: Add the flashed word picture, made realities striking into the correspondence. Attempt to include some particular realities and figure attempt to appealing the message beneficiary. Some more can place the activity into your action words. Lucidity: when correspondence to somebody, ensure u are clear about your objective or message. Attempt to limit the quantity of thoughts in each sentence. Ensure your sentence is straightforward your significance. Right: Make you are fits to your crowd when you are in correspondence. End You need to apply a correspondence procedure on the off chance that you wish to convey officially inside an association. The correspondence procedure is the ideal guide toward to accomplish a powerful correspondence. Certain hindrances may introduce in the correspondence procedure and it might welcome the negative effect on the procedure. Coincidentally, you can get the input on the correspondence which have happen and it tends to be entre that future correspondence are improve. Fruitful and compelling correspondence inside an association originates from the execution of their correspondence expertise in the event that they follow the correspondence procedure. All individuals inside the association will keep away from the obstructions and can be improved. Correspondence aptitude is significant in light of the fact that it can keep away from an inappropriate message you send or get. 2.0 Introduction A decent correspondence between the organizations and the client is very significant. It might coordinate impact the fame of the item. So there are significant on which sort of publicizing will you decide to promotion your business. Select a correct media for one to convey your message is a significant advance to building up your deals and advertising plan. The correct media for one business might not be right for another. Dubway is going to dispatch at Ipoh region. Dubway is an American café, basically they sells sandwiches, plates of mixed greens, and individual pizza. Target market of Dubway at Ipoh will be the individuals who get up early can would like to purchase a solid breakfast rapidly. As the item administrator of the first Dubway of Ipoh, Im picking the techniques, the mediums and the vehicles that I would use to speak with our intended interest group to convince them to purchase our item. 2.1 Advertising medium Promoting medium is the media variable to conveys the sponsors message to the buyer. This is the fundamental association between the item or administrations and the client who ready to get it. Normally a plural term of media is depicting channel of mass correspondence. A typical promoting media will be magazine, radio, TV and paper. Case of other correspondence vehicles: standard mail, board, travel inflatable, airships, inflatables, intelligent, fax and satellite. 2.1.1 Print Media Print media is the best method to scattering the data of promotion to the majority. Case of print media: paper, flyer, pamphlet and magazine. A commercial in print media can arrive at a wide number of individuals in an offered geographic to get the data since it is high peruser contribution. All in all, print media is a somewhat better chances to get the perusers consideration. In any case, it will be not viable if your ad in a not evident page or plan. 2.1.2 Electronic Media Electronic media is the method of electronic transmission. Its incorporate TV, radio, web, CD-ROMs, DVD, film and so forth. Electronic media of web are boundless appropriation at practically zero expense. These are additionally in a lower hindrance to passage for content makers contrasted with traditiona

7 Time Management Tips for Academic Writers

7 Time Management Tips for Academic Writers Between a 12-credit course load and work assignments, the life of a studentâ€"whether part-time or full-timeâ€"can get stressful. But before you down that eleventh cup of coffee and spend yet another sleepless night trying to meet deadlines for academic writing assignments, here are seven time management tips for academic writers to make your semester less stressed.With the right kind of time management, your academic writing assignments can be less stressful. Photo by Kaylah Otto on Unsplash.#1. Establish a timeline and stick to itTheres really no way around itâ€"academic writing requires time and preparation. This is especially true if you are in advanced undergraduate classes or graduate school. Gone are the days when you could procrastinate on a writing assignment, stay up all night the night before the due date to get it written, then still manage a good grade on the result. Its simply impossible to pull of when advanced research is involved.When youre given an assignment that w ill be due in one month, first determine the steps that will be involved with that assignment. For most academic writing assignments, such as essays or research papers, these steps will include:A trip to the library or online research to find sourcesTime to read through the sourcesTime to take notes and cite the sources usedTime to outline the intended direction of your writing and develop a thesis statement/argument/hypothesisTime to write the rough draftFinding an editor or second pair of eyes to look over your workTime to complete the final draftTime to complete the list of references or sources in a standard formatDepending on the scope of your assignment, locating and reading through your sources could take anywhere from an hour to several weeks, so its best to get started on this part of the academic writing assignment as quickly as possible. Some professors also place parameters on the types of sources you use or the dates they were published, which can add additional time an d stress to this part of the process.If your academic writing project is a thesis or dissertation, obviously, there are many more steps involved than the basic ones listed above. However, regardless of the assignment given, its important to plan out the steps that will be involved and establish a workable timeline that you can stick with.#2. Turn off your phone and social media accounts while writing/researchingWeve all been thereâ€"that moment when youre focused on a topic of study, or in the middle of writing, and your mind begins to move toward a deeper level of analysis. You begin to think clearly, the words flow smoother, and then suddenly…ding! A Facebook notification comes in and you lose it all.How much time did it take you to get to that place of focusâ€"the place you found before the notification distracted you? Ten minutes? Maybe more? Youre now back at square one, with muddled thoughts, and a looming deadline that requires you to once again find focus.When youre in the process of researching and writing, constant interruptions can cause more delay than you realize, because its more than those few seconds of interruptions. Its the added minutes required to once again focus your thoughts on the task at hand, and often, those minutes add up to hours if enough distractions are around you. This is especially true of social media, which can end up causing you to waste time on scrolling through yet more mindless memes and cute cat photos.#3. Outline your writingWriting without outlining is like driving a train with no tracks. To achieve a cohesive, well-written essay, you need an outline that gives you a general direction to take. It will help save time in the end by ensuring that your writing moves in the right direction.The time you should spend outlining your essay is dependent on the time you have to write it. For example, if its an in-class assignment and you have an hour to write it, obviously, you shouldnt spend more than 5 to 10 minutes on the o utline. However, if you have a month to pull together an academic essay assignment, you should spend several days creating an outline that will include all of the necessary elements the essay requires (e.g., introduction, thesis statement, body, and conclusion).If your academic assignment requires an abstract, your outline will be useful in writing it. The outline should contain an introduction to the problem, your thesis statement, and the points or hypotheses you plan to make. All of these will be necessary when composing your abstract.#4. Create your citations as you goAs you read through your sources and conduct research on the topic, go ahead and make a citation for each source used in the citation format your assignment requires. This is best done on note cards, placing the quote to be used on one side (along with the page number) and the full citation on the other. From this point, all that will be required is alphabetizing the sources used and typing them out in a references list. This is a great time-saving method and will cut a large chunk of time out of whats required to prepare the final draft before submitting your essay.To save even more time with citations, if while youre reading over your sources and you have access to the laptop youll be writing your paper on, go ahead and put the citations in a saved file. Some word processing programs will even put the citation in the correct format based on the citation style you choose. You can also use online resources such as Citation Machine to help with this part of the writing process.#5. Set a daily word count goal while writingAfter establishing a timeline for your academic writing assignment, you should know exactly how many days you have to work on the writing portion. Divide that amount equally by your word count requirement and make sure you reach your word count goal each day you write.For example, if your essay should be four to five pages long, double spaced and not including the citation por tion of your paper, that is approximately 1,200 to 1,500 words (300 words per page of double-spaced writing is a good estimate). If you have a week to research and turn in your assignment, set aside a day for research and gathering sources, a day for outlining and reading over sources, three days to write (with a goal of hitting 500 words a day), a day to have someone proofread and edit, then a day for you to wrap up the final draft.#6. Take breaks away from the screen and booksWhile taking breaks might seem counterproductive to saving time, it isnt. The strain of staring at small text in your source material and the bright light of a screen creates added tension that is best avoided by taking consistent breaks away from your research and writing work. Not doing so can produce headaches, blurry vision, eye strain and trouble sleepingâ€"all of which could potentially affect your ability to work and think clearly the next day.If possible, be sure to take these breaks by going outside and breathing in fresh air. If you cant manage to get outside for a few minutes, other healthy options are meditating, closing your eyes and listening to music, a yoga flow, and holding a plank position for several minutes to reduce stress on the lower back.When writing and researching, be sure to take these breaks by going outside and breathing in fresh air. Photo by Joshua yu on Unsplash.#7. Plan ahead for someone to check your workYou can spend days writing and preparing your academic paper, but a few misspelled words or grammar errors can end up costing you a passing grade on your work. Thats why you dont want to rely on software to check your work for errors.While your word processing program and online programs such as Grammarly might seem like a good option, particularly when youre pressed for time, evidence shows that they fail to catch even simple grammar and spelling errors.If you cant pay a professional editor to edit your work, many universities offer peer editing servic es in computer labs, libraries, or some other convenient spot on campus. Often, these services are free to students, but may require you to schedule an appointment in advance. If this is the option you end up taking, on the day you receive your assignment, be sure to call ahead and find out how to schedule a peer review session of your paper and leave yourself an extra day after that review to make any changes to the rough draft.However, you manage to do it, always have a second pair of eyes look over your writing before you turn it in as a final draft. Sometimes, its easy to miss even the most blatant grammar and spelling errors after focusing on one document for a long time. And no amount of research or preparation will make an essay full of typos an easy A.

Unsex Me Here Macbeth Analysis Essay - 642 Words

Scene Analysis: Macbeth Act 1 Scene 5 Act 1, Scene 5 is a soliloquy spoken by Lady Macbeth after she has read her husbands letter, and when she knows from the messenger that the king will be arriving that night. The raven himself is hoarse That croaks the fatal entrance of Duncan Under my battlements. Come, you spirits That tend on mortal thoughts! Unsex me here, And fill me from the crown to the toe top-full Of direst cruelty; make thick my blood, Stop up the access and passage to remorse, That no compunctious visitings of nature Shake my fell purpose, nor keep peace between Th effect and it! Come to my womans breasts, And take my milk for gall, you murdering ministers, Wherever in your sightless substances You wait†¦show more content†¦She then prays that they unsex her, in other words, she wants to be stripped of all her natural female emotions, tendencies and qualities, she wants to be deprived of any loving, maternal qualities, and to have them replaced by a direst cruelty. Lady Macbeth also pleads that they make thick my blood, Stop up the passage to remorse. Technically if her blood was made thicker, the blood flow throughout her body would be slowed down, and natural human functions would shut down. Metaphorically she wants the flow of remorse and pity to be cut off, and to be unhuman. However, it also shows that naturally she could be a decent person, because if she was all bad she wouldnt be pleading to dark forces to block up her consciece and pity. passage and access describe entrances, openings or routes. Lady Macbeth wants them all to be blocked so that she can commit any evil deed without remorse, so that no natural feelings can divert her from her original intent ions. (No compunctious visitings of nature). Lady Macbeth pleads that her breast milk be turned to bile. A womans breasts have the natural function to feed, and is a source of life for a growing baby, while bile is a bitter, foul green coloured substance made in the liver which provides no nourishment. This again links back to the central theme of the reversal of nature. Lady Macbeth also pleadsShow MoreRelatedPsychoanalytical Criticism of Macbeth1170 Words   |  5 Pagescharacter Lady Macbeth in William Shakespeare’s play Macbeth. When Macbeth was written in the beginning of the seventeenth century, masculine traits included valor, authority, and undaunted aggression while femininity was related to virtue, compliance and obeying her husband’s desires—the phrase: yes, my Lord was extremely common. In this sense, Lady Macbeth’s conduct must adhere to the strict codes of femininity placed before her by cultural injunctions of the period. 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The Problem Of The Energy Sector - 1930 Words

Among the sixteen critical infrastructures within our country, comes the fact that each and every one of them plays a very important role within keeping our nation up and going. Not only on an economical aspect but also in a safety aspect. Each of the sectors are equally as important as the next. The Dam sector is one of my favorites to talk about because I feel as though it is very much not thought about as a critical part of our infrastructure and I believe that there are many security holes within that particular sector. Today however, I will focus on the energy sector which I also see as not having serious constant talk about the threats that are a reality. When one thinks about the energy sector, many typically think about power lines and how it would cause an issue within a centralized location. This in fact is not all. Our Energy infrastructure actually involves: electricity, oil, and natural gas (Energy Sector). In recent years there have been more public acknowledgmen ts made in regards to the reality and severity of the threats to our electric power grids. Often times this is being referred to as a â€Å"Black Sky Day† (Threats) because it would be devastating. Professor Dr Daniel Baker, has testified before a couple of subcommittees of the US House of Representatives Committee last September and he shared the same concerns that were also shared by R. James Woolsley, who happens to be the chairman for the Foundation for Defense of Democracies. â€Å"During thatShow MoreRelatedUnemployment And Its Effects On Society1294 Words   |  6 Pages Problem Statement Unemployment is a very important problem that it is not only an economic issue, but it is related to the social and political issues. Increasing the level of unemployment will decrease the level of Keynes’ effective demand that is going to increase the investors’ uncertainty and less investments will take place. 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Animals in Captivity Free Essays

string(50) " animals such as penguins that live in captivity\." The zoo is packed with children, running everywhere. They laugh and smile as they watch the animals at the zoo sleep. What these children do not realize is that these animals are dying on the inside. We will write a custom essay sample on Animals in Captivity or any similar topic only for you Order Now Animals that live at the zoo are extremely depressed. These animals can suffer severe psychological disorders from being out of their natural environment. But others argue that keeping these animals in captivity will help keep endangered species alive. However, the disadvantages of keeping animals in captivity are becoming more and more serious, and more people are beginning to believe that animals should not be held captive. Animals should not be kept in captivity because of the negative impact it can have on their life. On Christmas Day, in the year 2007 a tiger broke out of its enclosure at the San Francisco Zoo. Once out of its cage, the tiger attacked 3 people, severely injuring two people, and killing one. Unfortunately, this was not the first time that this tiger had shown aggression towards people. A year before this incident, this tiger had injured a zoo keeper during a public feeding (Roberts, 2008). In captivity animals are isolated from their natural habitat, and are provided with very little physical and mental stimulation. According to the Association of Zoos and Aquariums (AZA), facilities with potential dangerous animals, must have appropriate safety procedures in order to prevent attacks by these animals. Sadly, these procedures were not followed at the San Francisco Zoo (Roberts, 2008). Another incident like this occurred with a killer whale and its trainer. Dawn Brancheau was drug to the bottom of her killer whale’s tank at Sea World in Orlando, Florida. Although these whales are called, â€Å"killers† there is no record of them killing human beings in the wild. According to the Whale and Dolphin Conservation Society, attacks by killer whales in captivity happens more than you think (McCarthy, 2010). Why do these animals attack in captivity and not in the wild? The answer to this question is believed to be linked to captivity related stress. In the wild, these animals are very social, living with 10 to 20 other whales. Placing these animals in captivity alters their behavior, and leads these animals to have unpredictable anger. Because of this animal welfare campaigners believe that killer whales should not be placed into captivity, but you can not just place the animals that were so easily caught into the wild again (McCarthy, 2010). People argue that keeping these kinds of animals in captivity provides the world with numerous educational benefits, but accidents like this are happening more and more. These incidents make a person wonder if these animals were never to be placed into captivity, would these tragedies have happened. Animals should not be kept in captivity because it has been proved to have a negative impact on their lives. Generally, animals that live in zoos suffer from poor well-being. Although, these animals receive veterinary care that they normally would not receive in the wild, they can suffer from severe stress. Evidence has been found that psychological needs of these animals are not being met. Not only do these animals suffer mentally, but they also suffer physically too. After being placed in captivity, some animals can become susceptible to opportunistic infections such as jaw abscesses (Mason, 2009). Also the giraffe has a short lifespan in captivity then it would normally have in the wild. The reasoning behind this is that they have low energy intake in the wild and poor nutritional status (Mason, 2009). Many animals that live in captivity do not seem the flourish in the same way that they would in the wild. Many animals that live in captivity do not get the kind of exercise that their bodies need, especially elephants (Smith, 2008). Being locked up in a facility might be helping these animals avoid poachers, but the lack of exercise is causing cardiovascular disease. Elephants that live in captivity do not live nearly as long as they would in the wild. 7 elephants were examined at a British zoo, and only 11 of them were able to walk correctly. It is said that advancements are being made to improve elephant environments in captivity, but numerous zoos have shut down their elephant attractions (Smith, 2008). Many people are attracted to keeping wild animals as pets. They believe the wild animals to be interesting and exciting. At a young age, the animals may seem easier to tame, but the older these animals get, the more aggressive they tend to become. Many problems can develop from keeping a wild animal as a pet. Wild animals have specific needs that have to be met, in order for them to prosper. â€Å"Only the most exceptional zoos and wildlife centers provide a living area that somewhat resembles the natural habitat of these animals, but it is virtually impossible to provide sufficient space for larger species†(CFHS). In captivity animals do not lose their wild instincts. They can be extremely unpredictable, and if provoked they can cause severe harm to people. Some wild animals, such as reptiles and hedgehogs can actually carry bacteria called salmonella, which is very easily transmitted to humans. Exotic animals can be very social, and need to have a companion of the same species living with them. If the animal is kept isolated from its kind, then it can suffer psychologically (CFHS). Many wild animals that are kept as pets often get abandoned because the owner was not able to meet of its needs. Others try to place the animal back into its natural habitat, but after being in captivity for many years, the animal is not able to re-adapt to this environment. When these animals are abandoned, it is difficult to find a new home for them. Sadly, most of these animals end up being humanely euthanized, or die from stress of being moved from one environment to another (CFHS). There are strict guidelines for some animals such as penguins that live in captivity. You read "Animals in Captivity" in category "Papers" In some areas of the world, it is actually illegal to hold these animals captive. Due to some of the elements that penguins are exposed to in captivity, some can become very ill, or even die (Penguin Facts, 2009). Although, a lot of these facilities are cleaned regularly, the illnesses can spread extremely fast to members of the penguin colony without warning. If the illness continues to spread throughout the colony then the penguins are all at risk of becoming ill, or even possibly dying (Penguin Facts, 2009). When new members are added to a colony, penguin may feel the urge to migrate, which is an instinct they must ignore in captivity(Penguin Facts, 2009). In zoos, it is commonly seen that two males or two females will have a relationship, but in the wild this is not seen (Penguin Facts, 2009). Another animal that does not do well in captivity is the monkey. Behind bars these animals are well fed and safe, but they will never be able to socialize with other types of monkeys like they normally would be able to in the wild. Victor Hugo explains, â€Å"People think they can tame these wild creatures because they’re so cute when they’re babies – but they inevitably bite someone and then become a problem†(Macaskill, 2011). Monkeys are believed to be cute and cuddly creatures, but this animal can actually feel threatened by this kind of attention from a human. Eventually, leading to attacking what it feels threatened by, as it would do in the wild. According to Victor, â€Å"Every day a monkey spends in captivity makes a difference and once they’ve become too humanized, they become non-releasable† (Macaskill, 2011). An argument many have placed in the defense of zoos is educational benefits and conservation. Not all zoos are bad. According to Michael Hutchins, PhD, director and William Conway Chair of the Department of Conservation and Science for the American Zoo and Aquarium Association, there are two kinds of zoos, zoos that meet AZA standards and zoos that just aren’t up to par (Keuhn, 2011). He also discussed that in the past few years, zoos have changed from focusing on preserving species by captive breeding to preserving habitats and species that live in the wild. Zoos support conservation by educating members of society, and raising money for conservation projects. They can also help develop technologies, and with scientific research (Keuhn, 2011). It is said that by observing animals in captivity, scientists can find valuable information that they would not be able to gather in by observing animals in the wild. However, conservation is not justification for zoos. Dr. Hutchins says that animal welfare is equally important. The AZA has taken a number of steps to improve animal welfare. They must promote natural habitats, and ensure quality care of their animals. They have also outreached to substandard zoos, to help close down the worst facilities (Keuhn, 2011). Some people argue that animals do not have rights. They believe that in order to keep endangered species alive, the animals must be captured. Zoos can also provide animals with safety from poachers and wildlife predators (Nakate, 2010). Some zoos do treat animals in a harsh manner, but there is improvement being made in the quality of care being provided to animals. There are many educational benefits that zoos and conservation centers provide people with. These places are trying to make more people aware of the environment (Nakate, 2010). Many schools take field trips to zoos in order to educate children, early in their lives about animals, and their environment. Teaching children about the environment, at a young age will help raise awareness about environmental issues later on in their lives. Terminating all zoos would hinder knowledge about some animals. For many scientists, it is hard for them to get a good look at animals in the wild. By placing some of these creatures in captivity, it enables scientists to get a closer look at these species and their behavior. Without zoos, conducting research would become a hassle. Scientists would have to go into the wild for several days to track the animal down, then once found, they would have to try to observe from a safe distance. Most penguins seem to do fairly well in zoos, and conservation centers. Captivity is beneficial to penguins that have been injured in the wild, and would have died without the help from animal caretakers (Penguin Facts, 2009). In the wild, penguin eggs have the chance of being destroyed by predators, but in captivity there is a chance for all the eggs to survive. Also, this gives animal caretakers a chance to help feed the baby penguins that the adults will not care for (Penguin Facts, 2009). Ultimately, captivity is very beneficial to the penguin population. Although, it may seem like zoos and other conservation centers provide people with educational benefits that is not always the case. Most children, who visit the zoo, do not even read the informational guides that are placed at each exhibit. Generally, people spend a few moments at each display, to take pictures of the animals then move on to the next without even taking a glance at any information given. While, not all zoos are bad, many zoos out there do not take proper care of their animals, leading them to a lifetime of misery, and pain. Without proper care, animals cannot function normally. Animals that do not live in their natural environment do not get the right amount of socialization that they need. This can cause severe physiological effects on them. The stress of moving animals from one environment to another can be harmful as well. Even though these animals are captive, they are still wild animals, and have natural instincts to protect themselves. If an animal in captivity believes it is in danger, it will attack a human, causing severe injury, or death. Some animals in captivity will attack just because it is in their nature. Once placing an animal in captivity, it cannot be placed back into its natural environment. Animals have a hard time re-adapting to the wild. Placing animals in captivity has its obvious benefits, but do these benefits outweigh the disadvantages that it has on an animal? When placing animals in zoos, people are not thinking about the natural well-being of them. They are only thinking of the scientific benefits and entertainment values that these animals bring to the world. Keeping animals in zoos may help out endangered species, but they will never be able to prosper outside of the cages of those facilities. Is an animal truly an animal when it’s trapped behind bars? How to cite Animals in Captivity, Papers

Canadian Public Policy-Free-Samples for Students-Myassignmenthelp

Question: Discuss about the Canadian Public Policy. Answer: Federation and public health administration in Canada One of the most critical issues in Canada history on federalism has been the issue of how public health has been administered since the 1950s. Although there has been an evolution of thus delivery in public health, its not yet to the standards. The Canadian government is divided into federal government and provisional government. Since Canada's 1867 constitution appointed most health care services obligations to commonplace jurisdiction,8 Canadian wellbeing strategy is inseparably interwoven with government common connections.(canada government, 1982) Canada is a league of 10 areas in addition to 3 scantily populated northern regions. These areas fluctuate hugely in both size and monetary limit, extending from the Atlantic territory of Sovereign Edward Island, with a 2001 populace of 135 000, to the mechanical heartland of Ontario, with 11.4 million. (SG, 2013) The countries policies concerning public health care have to greater extends affected how the administration and the delivery of these health care services are done. This has posed a lot of challenge. The Canadian constitution provided two levels of government and each level has its powers. The operations under these powers have made an impact in the healthcare sector. The provincial level of government has been set with more power when it comes to the public health administration policies in the establishment, maintenance and the management of hospitals and any public related non-governmental and governmental bodies.(soroka, 2007) Perspective related to this topic Considering the subject matter and topic of discussion, it would be relevant to state that from a public health perspective, administration and delivery of public health services can be greatly be affected either positively or negatively by the federation. Effect of the perspective on the issue This perspective has made me believe that the government and federation can help solve challenges that the health sector is facing in Canada. The fragmentation of the Canada government has made it easier to allocate resources even by the national government to help fund the activities of the provincial government in public health maintenance. Recommendations on what can be changed in Canadian public policy One way to help the situation in Canada is employing measures that entail the integration of banks and insurance companies with the health sector. This will help out the citizens of this country ease the burden of health costs since the government cannot reach individual needs at once. Moreover, the sector should ensure that they are working on reducing the wait time and improvement of access. It's true that too many Canadians wait in long line to be attended to by health professionals. This means that there are few doctors and nurses and therefore the relationship between government and health sector should come in such situation, employ more specialists and offer suitable compensation terms to them. In addition to that is the improvement of long-term care. Another recommendation is to call upon the government to invest its time, ideas, and finances in the health sector. The labor movements should partner with community groups in working in health care matters at all levels of the l eadership.(congress, 2015) Works Cited canada government. (1982). the constitution acts. congress, c. l. (2015, March 2). canadian labour congress. Retrieved 11 28, 2017, from ways to improve health care Social and Economic Policy Healthcare: https://canadianlabour.ca/issues-research/6-ways-we-are-working-improve-health-care SG, M. (2013). Rethinking Pharmacare in Canada. . Howe Institute. soroka, S. (2007). Health Council of Canada. Canadian perceptions of the healthcare system:. a report to the Health Council of Canada.

Barriers to an Effective Organizational Learning

Of late scientists, management scholars have become interested on the benefits that organizational learning has on an organization; they are of the agreement that an organization that constantly coaches, trains, mentors and counsels its employees is likely to have an edge of competitiveness in every phase of business (Hornsby and Warkeoczeski, 2000).Advertising We will write a custom assessment sample on Barriers to an Effective Organizational Learning specifically for you for only $16.05 $11/page Learn More Learning in an organization improves employees’ productivity; it increases the personnel expertise, which in turn keeps a company competitive; however, learning faces a number of challenges. This paper analyzes the major impediments to effective organizational learning (OL). Barriers to an effective OL Barriers to an effective OL are factors that hinder the implementation of OL programs within an organization or they at least interfere with th e practicability of the set programs; they impede the implementation of the change that learning is likely to bring out. Organizational culture is the first impediment of OL if it has not been well managed; an organization with a negative organizational culture is likely to see a learning process as a disruption of the organizational status quo and is likely to repel against it. In most case, organization learning comes with a change that the organization will go through, if the organizational culture adopted in an organization does not support a change process, then learning in such organization is difficult. Another hindrance of organizational culture is an organization politics; in every organization, some internal politics that determine how things are done; these politic are not formal however, they have the ability to create attitude, perceptions and behavior within an organization. If the politics of an organization are not positive to learning, then the process is likely not to be successful (Schilling and Kluge, 2009). Leadership within an organization plays a crucial role in the success of OL, leaders are seen as the pioneers of OL and they are constantly undertaking the process. When a company’s leadership is not effective and lack the expertise and knowledge to pioneer OL, the external processes are likely to influence the change.Advertising Looking for assessment on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More Different employees understand things differently; leaders should be sensitive enough to know the kind of employees that they have then come up with the right approach. In most cases, especially when it comes to training, the entire human capital cannot undergo the training since it might be very expensive to the company; however, some few employees are trained, and expected to diffuse the information and knowledge attained to other people. The external environment that a company is operating in is likelihood impendent of organizational culture; the external environment includes competitors, the culture of the people around and organization and the relationship that prevail between people and the organization. Organizations need to recognize that external factors/forces affect learning and knowledge development in the organization. To ensure that the effect is not negative, an organization needs to have good relationship with the external environment. To create good environment, a company need to be ethical in its processes and adopt corporate social responsibility activities; this will enhance the relationship that the company have with the external environment (Meinolf, Ariane, John and Ikujiro, 2003) Conclusion The success of organizational learning is hindered by internal and external factors facing an organization if the factors are not managed appropriately. The internal factors include organizational culture, organizational politics, leaders hip and informal team in the organization. The external factors likely to hinder organizational learning are corporate governance and culture of stakeholders, customers and the public. References Hornsby, T. and Warkeoczeski, L. 2000. New roles for leaders: A step-by-step guide to competitive advantage. Franklin: Hillsboro Press. Meinolf, D., Ariane, A., John, C. and Ikujiro, N.,2003. Handbook of Organisational Learning and Knowledge. Oxford: Oxford University Press.Advertising We will write a custom assessment sample on Barriers to an Effective Organizational Learning specifically for you for only $16.05 $11/page Learn More Schilling, J. and Kluge, A.,2009. Barriers to organizational learning: An integration of theory and research. International Journal of Management Reviews, 11(3), pp. 337–360 This assessment on Barriers to an Effective Organizational Learning was written and submitted by user Cruz U. to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Definitions and Examples of Partitives in Grammar

Definitions and Examples of Partitives in Grammar   In English grammar, a partitive is a word or phrase (such as some of or a slice  of) that indicates a part or quantity of something as distinct from a whole.Partitive is also called partitive noun or partitive noun phrase and is from the Latin partitus, relating to a part. Partitives can appear before mass (or noncount) nouns as well as count nouns. Although most partitive constructions refer to a quantity or amount, some are used to indicate quality or behavior (the kind of teacher  who ... ).  Ã‚   Examples and Observations You must have been warned against  letting the golden hours slip by. Yes, but some of them are golden only because we let them slip. (J.M. Barrie, Courage. Rectorial Address delivered at St. Andrews University, May 3, 1922)Computers make it easier to do a lot of things, but most of the things they make it  easier to do, dont need to be done. (Andy Rooney on 60 Minutes)Now Murrells eyes followed an ant on a blade of grass, up the blade and down, many times in the single moment. (Eudora Welty, A Still Moment. The Collected Stories of Eudora Welty. Harcourt, 1980)Soap gumdrops, soap cigars, soap pickles, soap chocolates, and even a bar of soap that dyed its user an indelible blue made life exciting for the friends of a Johnson Smith addict. (Jean Shepherd, A Fistful of Fig Newtons. Random House, 1981)Not a part of the rock or a speck of moss or a streak of some other mineral, it was one of those stubborn bits of green felted cardboard that these rocks were always fixed on inside of the boxes. (Sharon Fiffer, Buried Stuff. Minotaur Books, 2010) It doesn’t matter if you’re a high school kid on your bike, or if you’re an egghead like me with a  boatload  of degrees. Anybody can be a birder. (Ben Kingsley as Lawrence Konrad in A Birders Guide to Everything, 2014)I am not just some here-today-gone-tomorrow  sort of person who blows hot and cold  like a feather in the windblown about by air. Oh no. Believe me, my love for you is, was and always will be true and oh-so-real. (Dawn French, Dear David Cassidy in  Dear Fatty.  Arrow Books, 2009) Partitives With Count Nouns and Noncount Nouns Count nouns that can act as the first element in such a structure (e.g. piece, bit, sort, etc.) are partitive nouns or partitives. Some words that form the second part of the construction take specific partitives (also called unit nouns) a Partitives are useful because they provide a means of counting uncount nouns.(Sylvia Chalker and Edmund Weiner, Oxford Dictionary of English Grammar. Oxford University Press, 1994) Partitives With Nouns of Location and Time Partitives are  found with nouns of location (the end of the street, the back of the house etc.) and time (the end of the day, the middle of the week, the beginning of the month). These partitives of location and time are almost always found with the frame the partitive of the noun. (Dave Willis,  Rules, Patterns and Words: Grammar and Lexis in English Language Teaching. Cambridge University Press,  2003)  One day toward the end of the  month the wind veered around  to the southwest again and clouds moved in, bringing with them a heavy downpour. (John Hanson Mitchell,  Living at the End of Time: Two Years in a Tiny House.  University Press of New England, 2014)   Partitives With Foods and Liquids Some partitives, such as gallon/liter of, can be applied to any head noun that is a liquid, and partitives such as ton/gram/pound of can be used to quantify anything that is appropriately measured by weight. Similarly, partitives such as a bottle of can be applied to different types of liquids that come in this container (e.g., beer, wine, catsup, milk). In contrast, partitives used to quantify food are more restricted. Portions of baked goods such as cake, pie, pizza, and bread are measured by slices, and only bread is quantified by the partitive count noun loaf. Certain types of vegetables (e.g., cauliflower, cabbage, lettuce) are quantified by head.  (Ron Cowan, The Teachers Grammar of English: A Course Book and Reference Guide. Cambridge University Press, 2008)  The pub is very smart  and popular with foreigners, who can order Leopold Blooms lunch- a gorgonzola sandwich and a glass of burgundy- for about fifteen dollars during the summer high season. (Bill Barich,  A Pint of Plain: Tradition, Change and the Fate of the Irish Pub.  Bloomsbury, 2010) Functions of Partitives Partitive expressions collocate strongly with particular non-count nouns: a​ ... Partitive expressions commonly refer to the shape, size, movement or the amount of something: Theres a whole ... Some partitive expressions with -ful refer to containers or spaces which commonly hold the item referred to. These include bowlful of, cupful of, fistful of, handful of, mouthful of, spoonful of: He gave me a The plural of such expressions is usually formed by adding -s after -ful.(Ronald Carter and Michael McCarthy, Cambridge Grammar of English. Cambridge University Press, 2006)

Critical Analysis Paper Assignment Example | Topics and Well Written Essays - 1250 words

Critical Analysis Paper - Assignment Example Many of the major poles in this newly emerging multi-polar world will not be single nations, but rather clumps of nations who will exert a significant say as to how the new world order need to be contrived. In such a scenario the ubiquitous West is expected to lose its grip over the world economy and politics. In a more pragmatic sense the so called ubiquitous ‘Western Liberal Democracy’ is losing its charm as the history is set to evince major corrections over the next five years. The other remarkable thing that one would discern in the world politics will be the augmenting role of the non-state actors in the configuration of international relations and in the shaping of local and international political setups. The multinational corporations whose domains extend beyond the borders of the nations they ascribe to are poised to adhere to potent corporate foreign policies that are bound to configure and shape international relations in a variety of ways. The power of the international intergovernmental institutions and powers like the European Union and nongovernmental organizations like the international financial institutions are set to play a major role in the international polity and economy over the next five years. The nation states will stand vulnerable to the agenda of these intergovernmental and nongovernmental organizations and institutions. In the next five years the policy frameworks and governance agendas of the non-state actors will gain much prece dence amongst the developed and developing nations as the framework of these non-state actors are expected to become more inclusive and democratic. For example say the emergence of the international credit rating agencies and the influence that their predictions have on the flow of capital in the international markets is one way one could grasp the emerging power of the non-state actors. In the years to come, the important question will not as to how to dilute and diminish the role

Individual innovation appraisal- Concise written description of an Essay

Individual innovation appraisal- Concise written description of an innovation- proposal and evaluation - Essay Example For the innovation to existing in the market, availability of capital and implementation of sustainable market strategies will play a great role. Through proper planning, goal-setting a decision-making, the innovation will go through thorough market testing. After its viability is established, it will be released into the market. Another aspect that has contributed to its existence is availability of resources including human and financial resources. Without resources, it is impossible to get through with any innovation. Over the years, 3D has acquired popularity with more people being attracted to this kind of technology. The situation has forced entrepreneurs to develop new business models. In recent times, most companies are raising capital towards tapping the growing demand and market for 3D (Taylor, 2014). The aim maintaining relevance drives them. The Mink is triggered by the level of profitability expected. The Mink is likely to be successful mainly because of the much hype it has received from the general population. People are always attracted by unique and new ways of doing things. People also like to experiment even with things that they do not necessarily need. Marketing strategies that have been adopted to market The Mink will also ensure this success. Through advertising, the innovation is receiving popularity with more people embracing the idea every day. It is evident that advertising influences the success of a business to a great extent. Through various exhibitions, awareness has been created regarding The Mink. Social media has also played a role in enhancing the popularity. The innovation is, therefore, likely to succeed. Furthermore, it is a common believe that women are impulse buyers; profits are likely to increase since they are the main users of the innovation. Nonetheless, the cosmetic industry is doing well in the business market (Restauri, 2014). Most women use

DNA Transformation in Bacteria

DNA Transformation in Bacteria 1.0 Introduction and Objectives The ability of bacteria to incorporate DNA from external sources is the primary reason for their survival and proliferation. Bacteria can take DNA from their surroundings or from other bacterial cells by cell wall-transfer. While an interesting phenomenon to examine for scientists, practically it is of great concern for the human race and a source of constant challenge for the Pharmaceutical Industry. The ability of bacteria to modify their genetic information has given rise to problems such as antibiotic resistance wherein bacteria become resistant to medications that were once effective in eliminating them. In this experiment, we examine the development of antibiotic resistance in bacteria. Circular DNA called plasmids are introduced in bacteria whose cells have been modified to promote uptake of plasmid DNA. This plasmid DNA will give rise to antibiotic resistance in the bacteria, which can be observed by allowing the bacteria to proliferate in an environment containing the antibi otic. Modification of genetic information in bacteria may be a source of concern, but that ability in the hands of humans has always been coveted. Genetic engineering is an increasingly popular research area given the breakthroughs made in recent years and the potential for commercial application. Various applications require large quantities of specific DNA sequences and this is where the bacterial ability to uptake DNA and reproduce it is beneficial. Introducing plasmids containing desired sequences into bacteria, allowing bacteria to reproduce and then isolating the required DNA is a common method used to obtain large quantities of particular DNA sequences. This aspect is also explored in this experiment. 1.1 Objectives The objectives of this experiment are to: a) Observe and examine the phenomenon of DNA Transformation. b) Observe the development of antibiotic resistance in bacteria through the process of gene transformation. c) Inculcate proper Sterile Technique for laboratory procedures involving bacterial strains. 2.0 Principles This section explores the underlying concept behind the experiment. Genetic Transformation is a process of horizontal gene transfer whereby DNA from the environment is taken up by a host cell. In this experiment bacterial cells are transformed. Escherichia Coli bacteria, which are generally non pathogenic are used in this experiment. The plasmids which constitute the external DNA contain a gene that makes the cell ampicillin resistant. Ampicillin is a bacteriostatic and will normally prevent the reproduction of E. Coli bacteria. This provides us with an easy way to test if gene transformation has occurred and to what extent by means of calculating the transformation efficiency. The introduction of genetic material within the bacterial cell is done by the process of electroporation. Electroporation involves applying an electrical voltage across the bacterial cells containing the plasmids. The ionic concentration of the DNA is kept low to prevent arcing. When the voltage is applied, holes open up in the walls of the bacteria. The plasmids can then enter the bacterial cells through these holes. Application of the voltage is done for a very short period of time. As soon as electric current stops flowing, the holes in the cell wall begin to close. A nutrient rich medium is then added to the bacterial cells, some of which will have transformed, to aid cell recovery. Incubation is then carried out, after which the cell suspension is diluted further and applied to agar plates containing the antibiotic. The cells are left to incubate for up to 24 hours and then the number of colonies determined. Calculating the transformation efficiency gives us a method to determine the extent to which the transformation occurred. 3.0 Methods and Materials 3.1 Materials The equipment and materials required for this experiment are outlined in this section. Equipment Required: A shaking incubator operating at 37ÃÅ'Ã…  C A non-shaking incubator An electroporator Materials Required: Cells treated for competency 2 agar plates with ampicillin with a concentration of 100 Â µg/ml pUC-19 plasmids 0.1 cm cuvettes Ice in an ice-box Deionised ultrapure water S.O.C. medium at room temperature 2 tubes with snap caps with a volume of 15 ml 3.2 Sterile Technique Sterile Technique is a must when handling pathogenic strains of bacteria. In this experiment, nonpathogenic bacterial strains are employed. However, using sterile technique is still good experimental procedure and promotes safety. Using sterile technique prevents errors in experimental results by preventing contamination from the surroundings. It also prevents contamination of the surrounding environment by the bacterial strain. Steps employed to prevent contamination included: Carrying out the experiment in an uncluttered area. Utilizing a fume hood to perform all procedures involving the bacteria. Washing hands both before as well as after the experiment Disposing off all bacterial waste in the appropriate container for bio-hazardous materials. 3.3 Procedure 3.3.1 Preparation for Electroporation The 0.1 cm cuvettes were cooled on ice. The electroporator was prepared based on prescribed settings. In order to bring the S.O.C. medium to room temperature, it was removed from the ice box. The cells and plasmids were allowed to thaw in the ice-box. Plates were heated at 37ÃÅ'Ã…  C to prepare for the incubation process. 3.3.2 Procedures I Â µl of pUC19 control DNA and 1 Â µl of ultrapure water were added to 2 separate microcentrifuge tubes with the aid of a pipette. The tube was then placed in the ice-box. 25 Â µl of competent cells were added to each of the microcentrifuge tubes. The contents of the tubes were gently mixed. Care was taken to avoid usage of the pipette for mixing. The tubes were then returned to the ice-box for 1 minute. The contents of each microcentrifuge tube were transferred to a cuvette using a pipette. It was ensured that the cells made contact with the cuvette walls and that no air-bubbles were present. This step was done rapidly to prevent heating up of the cells. The cuvettes were then electroporated. 250 Â µl of S.O.C. medium was added to the cells immediately after electroporation. Each of the two suspensions was transferred to a 15 ml tube. The shaking incubator was then set to 225rpm and used to incubate the cells for an hour to allow expression of the acquired antibiotic resistance. 10 Â µl of the transformed sample was then added to 90 Â µl of S.O.C. medium. The plates containing the ampicillin were then used. 20 Â µl of each of the two diluted samples from step 7 was added to a plate. Even spreading of the sample on the agar medium was ensured. Using the non-shaking incubator, the plates were incubated at 37 ÃÅ'Ã…  C for a day and the results recorded. 4.0 Results and Discussion 4.1 Results Answers to Questions (1) Schematic of observations of the agar plates: Figure 1: Results as Indicated by the Agar Plates (2) Count the colonies and calculate the transformation efficiency. Number of colonies observed = 13 Figure 2: Calculation of Transformation Efficiency Using the formula shown in figure 2, Transformation efficiency = 1.78 1010 transformants/Â µg plasmid DNA 4.2 Discussion Answers to Questions (1) Define the vocabulary used in this experiment: transformation, electroporation, host, plasmid, and competent. -Transformation Transformation is a process of horizontal gene transfer whereby DNA present in the environment of a cell is taken up by the cell. In this experiment the transformation involves the uptake of a plasmid containing a marker that results in ampicillin resistance by E. Coli bacteria through electroporation. -Electroporation Electroporation involves subjecting cells to an electric voltage to create holes in the cell wall. External material can then enter the cell through these holes. Natural processes then cause the hole to close and return the cell to its original state. -Host An organism that harbours a parasite is called a host. -Plasmid A plasmid is circular extra-chromosomal DNA. -Competent A competent cell is one which can internalise DNA present in its external environment. Competence can either be natural or artificial. (2) State why E. coli is used in many genetic engineering experiments. The popularity of Escherichia Coli for genetic experiments is due to various reasons. Firstly, most E. Coli strains are non-pathogenic and pose no harm to humans. Safety is a significant factor in the laboratory and E. Coli use is generally safe. Secondly, E. Coli grow easily and can be duplicated through metagenics. Thirdly, their genetic make-up is relatively simple and can be manipulated with ease. Fourthly they have been extensively studied and a lot is known about them. This makes it easier for researchers and they therefore prefer to use E. Coli for genetic engineering experiments. (3) Explain why competent cells, ampicillin, and S.O.C. medium were used for the transformation. Competent cells are necessary as transformation involves taking external genetic material into the cell. If cells are not competent this cannot happen and the experiment cannot be carried out successfully. Ampicillin is an antibiotic. Specifically, it is a bacteriostatic for E. Coli. It helps distinguish between bacteria that have taken up the plasmid and those that have not. This is because the plasmid contains a marker that causes ampicillin resistance. E. Coli cells do not naturally contain the genetic sequence that causes ampicillin resistance. Thus, ampicillin selection is possible to distinguish between transformed cells and untransformed cells. S.O.C. medium contains the nutrients required to help cells stabilise after electroporation. Electroporation introduces holes into the cell wall of the cell and therefore causes destabilisation of the cell. S.O.C medium contains yeast extract and other nutrient sources that help the cell recover. Once the cell has recovered and if the plasmid has entered the cell during electroporation, the cell will multiply and give rise to a colony during the incubation period. (4) Explain the purpose of the controls in this experiment. The control in this experiment constitutes bacteria without the plasmid that inculcates antibiotic resistance. Without this extra piece of genetic information to enable the bacteria to mount defences against the attack of the antibiotic, ampicillin is this case, the bacterial cells will be unable to multiply in a medium that contains the antibiotic. The cells that were treated such that they could incorporate the plasmid DNA into their genetic make-up will be able to multiply in a medium where ampicillin is present as long as there are enough nutrients available for growth. Thus, the control helps us show that the DNA plasmid was indeed taken up and incorporated into their genetic make-up by the bacteria. The only way for E. coli to have survived with ampicillin present is if they had taken up the plasmid and transmitted it to all generations when they reproduced after uptake of the plasmid. Hence, the control serves to confirm uptake of the plasmid as well as its transmission to fol lowing generations by comparing it to cells in the control that did not have the extra DNA. (5) Explain how the colony growth relates to gene transformation. A colony of bacteria stems from the binary fission of one single bacterial cell. When bacteria reproduce vertical genetic transfer occurs whereby the offspring has the exact copy of the genetic material of the parent. In this experiment, bacteria are introduced into a medium containing the antibiotic ampicillin. E. Coli bacteria with their original genetic make-up will be unable to reproduce due to the presence of the antibiotic as they do not have the means necessary to resist antibiotic attack. This is what is expected in the control sample as ampicillin is a bacteriostatic.. The positive sample on the other hand has bacteria which have undergone horizontal gene transfer by transformation. The plasmid DNA that was used for the transformation process contains genetic code that results in E.Coli developing ampicillin resistance. Thus, bacteria that can incorporate this plasmid and pass it on to their offspring by vertical gene transfer can grow in the environment. This is how colony growth relates to gene transformation. (6) Describe how ionic strength of DNA solution affects electroporation. The ionic strength of DNA solution comes into play due to the electroporation stage where holes are created in the bacterial cell wall to allow uptake of the plasmid by transmission of an electric voltage. For this step, the ionic strength of the solution must be low. If the ionic strength is high, arcing will occur. Arcing is visible during the experiment by sparks and a sound like a micro-scale thunderclap. It can cause cell death as well as equipment damage. Thus, for the experiment to be carried out successfully and to safeguard the apparatus, the DNA solution must be of low ionic strength. (7) If your transformation efficiency is lower than 1 109 cfu/ÃŽ ¼g, conjecture and explain potential reasons for the low efficiency. The transformation efficiency is greater than the benchmark stated above. This corresponds to good transformation efficiency and indicates a successful transformation process. However, the close clustering of the colonies makes it possible that some of the colonies are satellite colonies rather than transformed colonies. The experiment could be repeated with a higher concentration of ampicillin to obtain more reliable results. (8) Discuss current and potential applications of gene transformation techniques in biotechnology. Gene transformation techniques play a crucial role in biotechnology. This is because gene transformation provides a method to produce copies of desired DNA sequences. This is especially useful in the pharmaceutical industry to develop medications that are target specific. Also, this could potentially lead the way to genetic engineering, where defects to the genetic code could be repaired and desired traits inserted through addition of the corresponding DNA sequences. Gene replacement therapy could prove to be the cure for nearly all diseases that take human lives contemporarily. In the future gene transformation could be used to engineer human beings and other animals and plants according to desired specifications. Genetic transformation is also used in the development of pest-resistant crops, which could potentially increase the productivity of the land. This could be key to feed the ever-growing population as the quantity of agricultural land decreases. Understanding the evolution of drug resistance could help us devise ways of preventing drug resistance as well as developing drugs that can overcome resistance. In this arena gene transformation plays an important role horizontal genetic transfer is a natural process in bacteria. 4.3 Sources of Error and Suggestions for Improvement There are a few sources of error that could result in incorrect conclusion being drawn from experimental results. (i) The number of colonies seen need not correspond to the bacteria that transformed. This could be due to the growth of satellite colonies. Large bacterial colonies will secrete beta lactamase, which is what causes ampicillin resistance. Thus, the area around the colony will contain this secretion and be ampicillin-free. A satellite colony could grow in this area from untransformed cells. To avoid this problem, the incubation period should strictly be restricted to 24 hours. Satellite colonies emerge after a delay. By ensuring that results arr recorded promptly, the interference in results brought about by satellite colonies can be minimised. Another method is to use a higher concentration of ampicillin. More time will be required to create a antibiotic-free zone around a colony if the concentration of antibiotic is high. (ii) Identifying the number of colonies can be difficult, especially if the size of the colony is miniscule. This could result in an incorrect calculation of transformation efficiency. In order to increase accuracy of results, a different selection marker can be used. Some selection markers have properties that can be distinguished by shining UV light and other such techniques which result in a high contrast. Using these markers may result in higher reliability of results. (iii) Distinguishing between colonies can be difficult if they grow close to one another and appear to be one large colony. Also, closer colonies would also result in a higher chance of there being satellite colonies. To minimise this problem, crowding on the plate must be minimised. For that, a higher concentration of ampicillin could be used, carbenicillin selection could be used instead of ampicillin selection (although expensive) or the nutrient dilution could be adjusted such that it discourages very rapid proliferation. 5.0 Conclusions The objectives of this experiment were to explore the phenomenon of gene transformation and the development of antibiotic resistance in bacteria as well as to inculcate the practice of sterile technique for handling bacteria. Gene transformation was observed with the development of ampicillin resistance in transformed Escherichia Coli bacteria. The bacteria not exposed to the plasmids containing the genes for antibiotic resistance did not grow in an environment containing the antibiotic while the transformed bacteria formed colonies in the same environment. A calculation of transformation efficiency returned a value of 1.78 1010 transformants/Â µg of plasmid DNA, which is greater than the threshold of 109, indicative of a successful experiment. However, the possibility of some of the 13 colonies of bacteria being satellite colonies as opposed to transformed colonies reduces the reliability of the results. Methods to increase reliability of results were therefore suggested. References 1. Port, Tami. (2008, June 14). Bacteria Horizontal Gene Transfer. suite101.com. Retrieved 3rd April, 2010 from http://bacteriology.suite101.com/article.cfm/bacteria_horizontal_gene_transfer 2. Metzenberg, Stan. (2002). Bacterial Plasmids. California State University Northridge Department of Biology. Retrieved 4th April, 2010 from http://escience.ws/b572/L2/L2.htm

Significant Event

One of these events stands out in particular to me and had he greatest impact in my life. Physically disciplining a child has a dramatic impact on both the child as well as the parent. As a child I remember that for any little misbehaver resulted in a physical punishment as well as getting yelled at, as time went by my mind was set that I already knew when the â€Å"beating† was going to come. My family continue to be very big on respect and discipline, but at the time little did they know that by hitting us they weren't just punishing us for a little while, they were in fact pushing us away and causing frustration, at times even hatred.I also researched that in result of physically harming children increases their risk of mental illness, in which resulted when I came upon this article which states that â€Å"It's pretty well established that physically harming children has a negative impact on mental health, but this is showing the same effect even when you look at milder fo rms of physical force. This is saying that physical punishment should not be used on children of any age. † Each perspective has its strengths and weaknesses, which brings difference to our understanding of the human behavior.In my situation am going to go with the behavioral perspective, as well as the psychodrama perspective. By looking at the physical punishment from a behaviorism perspective, conditioning by pain requires that the consequence always occurs immediately after every incident. The psychodrama perspective states that in childhood certain incidents may occur that produce behaviors in their adulthood. Many different conflicts throughout childhood development shape overall personality. Observational learning refers to learning that occurs as a function of observing, and placating behavior observed in which is particular during childhood.I later figured out that the way my mother and aunts were physically punishing me, resulted in the fact that they learned from my grandmother, they completely mimicked the way my grandmother punished them. As I grew older I began to express myself to them in a matter that I felt curious I began to question why would they always physically harm my cousins as well as myself, their excuse still remains the exact same in which they say † That it is simply something they grew up seeing on a daily basis†. SST memories are not always accurate which can result in a blur, but do believe that it depends on how intense was the situation. There are many memories do vividly remember from my childhood but they are also plenty which don't necessarily remember everything, at times will looking at certain photos and remember exactly what I did that particular day, so I do think that it all just depends on the importance of the situation. Although do in fact accurately remember many different situations in which was hit, many of which I now think back ND know that could have been easily solved with just a simple co nversation.

White King, Red Rubber, Black Death

White King, Red Rubber, Black Death â€Å"White King, Red Rubber, Black Death,† illustrated how King Leopold II of Belgium acquired the Congo as a free state and exploited it by reign of terror. King Leopold II took over leadership of Belgium from his father, hoping to gain power and wealth, as well as assuming control of overseas territories like most other European nations of the time did. Leopold created, through political lobbying and military force, the Belgian Free State.The Belgian Free State gave Leopold power over the African territory of the Congo, which he soon exploited for its large supplies of ivory and rubber. Leopold and his soldiers used the Congolese natives as forced labor, and those who refused to work for the Belgians or who violated their newly established laws were punished by mutilation, torture, or death. Nearly ten million people were either slaughtered or worked to death in the Congo under Leopold's rule.In the end, Leopold's reign of terror in the C ongo became a scandal during the last years of his rule, and he destroyed most of the documents pertaining to the Belgian Free State prior to his death. Before this History class, I would have never had known about the documentary â€Å"White King, Red Rubber, Black Death† and about the many issues that were present in the Congo of Africa in the past. This documentary had left me speechless and astonished in the end. It had truly opened my eyes to the many tragedies that the Africans of the Congo endured under the reign of King Leopold II.Furthermore, I wondered, why throughout the entire reign of King Leopold II didn’t anyone even try bringing him down and making him pay for his cruel punishments depicted upon the Congo popularity? It amazes me that, during the thirty years that Leopold committed acts of violence and cruelty amongst the Congo, Leopold was still worshiped after by the Belgium’s as the man who colonized the Congo and made the Belgium city wealthy . This is a man who depicted the ignorance and greediness of Belgium.In my eyes, everything that Belgium has today, is out of fraud and lies because of the selfish acts Leopold committed. All of this cruelty, because of the exportation of rubber? I couldn’t believe it with my own eyes and ears, the amount of damage Leopold had caused amongst the Congo and its people just because of his obsession with power and wealth. Yet, if this was an issue that was being presented in today’s society of a specific area many would jump to fight and defend this area.King Leopold conducted many extreme measures in the past for no reason, in my opinion. If I was alive during this period and was one of the many people of the Congo who were enduring this cruelty and pain, I would stand up for what I believe in, save my people, and make a change. I do believe that if such person in today’s society, conducted these acts of punishment they would immediately get the death penalty becau se of the well improved justice system we have today, but why not back then?

All Roads Lead to the Ancient Network of Roman Roads

The Romans created a network of roads all across the empire. Initially, they were built to move troops to and from trouble spots. They were also used for speedy communication and ease of pre-motorized travel. Roman roads, specifically  viae, were the veins and arteries of the Roman military system. Through these highways, armies could march across the Empire from the Euphrates to the Atlantic. They say, All roads lead to Rome. The idea probably comes from the so-called Golden Milestone (Milliarium Aureum), a marker in the Roman Forum listing the roads leading throughout the Empire and their distances from the milestone. Appian Way The most famous Roman road is the Appian Way (Via Appia) between Rome and Capua, built by the censor Appius Claudius (later, known as Ap. Claudius Caecus blind) in 312 B.C., site of his descendant Clodius Pulchers murder. A few years before the (virtually) gang warfare that led to Clodius death, the road was the site of the crucifixion of the followers of Spartacus when the combined forces of Crassus and Pompey finally put an end to the slave revolt. Via Flaminia In Northern Italy, the censor Flaminius made arrangements for another road, the Via Flaminia (to Ariminum), in 220 B.C. after the Gallic tribes had submitted to Rome. Roads in the Provinces As Rome expanded, it built many roads in the provinces for military and administrative purposes. The first roads in Asia Minor were built in 129 B.C. when Rome inherited Pergamum. The city of Constantinople  was at one end of the road known as the Egnatian Way (Via Egnatia [á ¼ËœÃŽ ³ÃŽ ½ÃŽ ±Ãâ€žÃŽ ¯ÃŽ ± á ½â€°ÃŽ ´ÃÅ'Ï‚]) The road, built in the second century B.C., went through the provinces of Illyricum, Macedonia, and Thrace, starting at the Adriatic at the city of Dyrrachium. It was constructed by order of Gnaeus Egnatius, proconsul of Macedonia. Roman Road Markings Milestones on the roads give the date of construction. During the Empire, the emperors name was included. Some would have provided a place for water for humans and horses. Their purpose was to show miles, so they might include distance in Roman miles to important places or the end point of the particular road. The roads did not have a foundation layer. Stones were laid directly on topsoil. Where the path was steep, steps were created. There were different paths for vehicles and for pedestrian traffic. Sources Colin M. Wells, Roger Wilson, David H. French, A. Trevor Hodge, Stephen L. Dyson, David F. Graf Roman Empire The Oxford Companion to Archaeology. Brian M. Fagan, ed., Oxford University Press 1996Etruscan and Roman Roads in Southern Etruria, by J. B. Ward Perkins.  The Journal of Roman Studies, Vol. 47, No. 1/2. (1957), pp. 139-143.   A History of Rome to the Death of Caesar, by Walter Wybergh How, Henry Devenish Leigh; Longmans, Green, and Co., 1896.