Barriers to an Effective Organizational Learning

Of late scientists, management scholars have become interested on the benefits that organizational learning has on an organization; they are of the agreement that an organization that constantly coaches, trains, mentors and counsels its employees is likely to have an edge of competitiveness in every phase of business (Hornsby and Warkeoczeski, 2000).Advertising We will write a custom assessment sample on Barriers to an Effective Organizational Learning specifically for you for only $16.05 $11/page Learn More Learning in an organization improves employees’ productivity; it increases the personnel expertise, which in turn keeps a company competitive; however, learning faces a number of challenges. This paper analyzes the major impediments to effective organizational learning (OL). Barriers to an effective OL Barriers to an effective OL are factors that hinder the implementation of OL programs within an organization or they at least interfere with th e practicability of the set programs; they impede the implementation of the change that learning is likely to bring out. Organizational culture is the first impediment of OL if it has not been well managed; an organization with a negative organizational culture is likely to see a learning process as a disruption of the organizational status quo and is likely to repel against it. In most case, organization learning comes with a change that the organization will go through, if the organizational culture adopted in an organization does not support a change process, then learning in such organization is difficult. Another hindrance of organizational culture is an organization politics; in every organization, some internal politics that determine how things are done; these politic are not formal however, they have the ability to create attitude, perceptions and behavior within an organization. If the politics of an organization are not positive to learning, then the process is likely not to be successful (Schilling and Kluge, 2009). Leadership within an organization plays a crucial role in the success of OL, leaders are seen as the pioneers of OL and they are constantly undertaking the process. When a company’s leadership is not effective and lack the expertise and knowledge to pioneer OL, the external processes are likely to influence the change.Advertising Looking for assessment on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More Different employees understand things differently; leaders should be sensitive enough to know the kind of employees that they have then come up with the right approach. In most cases, especially when it comes to training, the entire human capital cannot undergo the training since it might be very expensive to the company; however, some few employees are trained, and expected to diffuse the information and knowledge attained to other people. The external environment that a company is operating in is likelihood impendent of organizational culture; the external environment includes competitors, the culture of the people around and organization and the relationship that prevail between people and the organization. Organizations need to recognize that external factors/forces affect learning and knowledge development in the organization. To ensure that the effect is not negative, an organization needs to have good relationship with the external environment. To create good environment, a company need to be ethical in its processes and adopt corporate social responsibility activities; this will enhance the relationship that the company have with the external environment (Meinolf, Ariane, John and Ikujiro, 2003) Conclusion The success of organizational learning is hindered by internal and external factors facing an organization if the factors are not managed appropriately. The internal factors include organizational culture, organizational politics, leaders hip and informal team in the organization. The external factors likely to hinder organizational learning are corporate governance and culture of stakeholders, customers and the public. References Hornsby, T. and Warkeoczeski, L. 2000. New roles for leaders: A step-by-step guide to competitive advantage. Franklin: Hillsboro Press. Meinolf, D., Ariane, A., John, C. and Ikujiro, N.,2003. Handbook of Organisational Learning and Knowledge. Oxford: Oxford University Press.Advertising We will write a custom assessment sample on Barriers to an Effective Organizational Learning specifically for you for only $16.05 $11/page Learn More Schilling, J. and Kluge, A.,2009. Barriers to organizational learning: An integration of theory and research. International Journal of Management Reviews, 11(3), pp. 337–360 This assessment on Barriers to an Effective Organizational Learning was written and submitted by user Cruz U. to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Definitions and Examples of Partitives in Grammar

Definitions and Examples of Partitives in Grammar   In English grammar, a partitive is a word or phrase (such as some of or a slice  of) that indicates a part or quantity of something as distinct from a whole.Partitive is also called partitive noun or partitive noun phrase and is from the Latin partitus, relating to a part. Partitives can appear before mass (or noncount) nouns as well as count nouns. Although most partitive constructions refer to a quantity or amount, some are used to indicate quality or behavior (the kind of teacher  who ... ).  Ã‚   Examples and Observations You must have been warned against  letting the golden hours slip by. Yes, but some of them are golden only because we let them slip. (J.M. Barrie, Courage. Rectorial Address delivered at St. Andrews University, May 3, 1922)Computers make it easier to do a lot of things, but most of the things they make it  easier to do, dont need to be done. (Andy Rooney on 60 Minutes)Now Murrells eyes followed an ant on a blade of grass, up the blade and down, many times in the single moment. (Eudora Welty, A Still Moment. The Collected Stories of Eudora Welty. Harcourt, 1980)Soap gumdrops, soap cigars, soap pickles, soap chocolates, and even a bar of soap that dyed its user an indelible blue made life exciting for the friends of a Johnson Smith addict. (Jean Shepherd, A Fistful of Fig Newtons. Random House, 1981)Not a part of the rock or a speck of moss or a streak of some other mineral, it was one of those stubborn bits of green felted cardboard that these rocks were always fixed on inside of the boxes. (Sharon Fiffer, Buried Stuff. Minotaur Books, 2010) It doesn’t matter if you’re a high school kid on your bike, or if you’re an egghead like me with a  boatload  of degrees. Anybody can be a birder. (Ben Kingsley as Lawrence Konrad in A Birders Guide to Everything, 2014)I am not just some here-today-gone-tomorrow  sort of person who blows hot and cold  like a feather in the windblown about by air. Oh no. Believe me, my love for you is, was and always will be true and oh-so-real. (Dawn French, Dear David Cassidy in  Dear Fatty.  Arrow Books, 2009) Partitives With Count Nouns and Noncount Nouns Count nouns that can act as the first element in such a structure (e.g. piece, bit, sort, etc.) are partitive nouns or partitives. Some words that form the second part of the construction take specific partitives (also called unit nouns) a Partitives are useful because they provide a means of counting uncount nouns.(Sylvia Chalker and Edmund Weiner, Oxford Dictionary of English Grammar. Oxford University Press, 1994) Partitives With Nouns of Location and Time Partitives are  found with nouns of location (the end of the street, the back of the house etc.) and time (the end of the day, the middle of the week, the beginning of the month). These partitives of location and time are almost always found with the frame the partitive of the noun. (Dave Willis,  Rules, Patterns and Words: Grammar and Lexis in English Language Teaching. Cambridge University Press,  2003)  One day toward the end of the  month the wind veered around  to the southwest again and clouds moved in, bringing with them a heavy downpour. (John Hanson Mitchell,  Living at the End of Time: Two Years in a Tiny House.  University Press of New England, 2014)   Partitives With Foods and Liquids Some partitives, such as gallon/liter of, can be applied to any head noun that is a liquid, and partitives such as ton/gram/pound of can be used to quantify anything that is appropriately measured by weight. Similarly, partitives such as a bottle of can be applied to different types of liquids that come in this container (e.g., beer, wine, catsup, milk). In contrast, partitives used to quantify food are more restricted. Portions of baked goods such as cake, pie, pizza, and bread are measured by slices, and only bread is quantified by the partitive count noun loaf. Certain types of vegetables (e.g., cauliflower, cabbage, lettuce) are quantified by head.  (Ron Cowan, The Teachers Grammar of English: A Course Book and Reference Guide. Cambridge University Press, 2008)  The pub is very smart  and popular with foreigners, who can order Leopold Blooms lunch- a gorgonzola sandwich and a glass of burgundy- for about fifteen dollars during the summer high season. (Bill Barich,  A Pint of Plain: Tradition, Change and the Fate of the Irish Pub.  Bloomsbury, 2010) Functions of Partitives Partitive expressions collocate strongly with particular non-count nouns: a​ ... Partitive expressions commonly refer to the shape, size, movement or the amount of something: Theres a whole ... Some partitive expressions with -ful refer to containers or spaces which commonly hold the item referred to. These include bowlful of, cupful of, fistful of, handful of, mouthful of, spoonful of: He gave me a The plural of such expressions is usually formed by adding -s after -ful.(Ronald Carter and Michael McCarthy, Cambridge Grammar of English. Cambridge University Press, 2006)

Critical Analysis Paper Assignment Example | Topics and Well Written Essays - 1250 words

Critical Analysis Paper - Assignment Example Many of the major poles in this newly emerging multi-polar world will not be single nations, but rather clumps of nations who will exert a significant say as to how the new world order need to be contrived. In such a scenario the ubiquitous West is expected to lose its grip over the world economy and politics. In a more pragmatic sense the so called ubiquitous ‘Western Liberal Democracy’ is losing its charm as the history is set to evince major corrections over the next five years. The other remarkable thing that one would discern in the world politics will be the augmenting role of the non-state actors in the configuration of international relations and in the shaping of local and international political setups. The multinational corporations whose domains extend beyond the borders of the nations they ascribe to are poised to adhere to potent corporate foreign policies that are bound to configure and shape international relations in a variety of ways. The power of the international intergovernmental institutions and powers like the European Union and nongovernmental organizations like the international financial institutions are set to play a major role in the international polity and economy over the next five years. The nation states will stand vulnerable to the agenda of these intergovernmental and nongovernmental organizations and institutions. In the next five years the policy frameworks and governance agendas of the non-state actors will gain much prece dence amongst the developed and developing nations as the framework of these non-state actors are expected to become more inclusive and democratic. For example say the emergence of the international credit rating agencies and the influence that their predictions have on the flow of capital in the international markets is one way one could grasp the emerging power of the non-state actors. In the years to come, the important question will not as to how to dilute and diminish the role

Individual innovation appraisal- Concise written description of an Essay

Individual innovation appraisal- Concise written description of an innovation- proposal and evaluation - Essay Example For the innovation to existing in the market, availability of capital and implementation of sustainable market strategies will play a great role. Through proper planning, goal-setting a decision-making, the innovation will go through thorough market testing. After its viability is established, it will be released into the market. Another aspect that has contributed to its existence is availability of resources including human and financial resources. Without resources, it is impossible to get through with any innovation. Over the years, 3D has acquired popularity with more people being attracted to this kind of technology. The situation has forced entrepreneurs to develop new business models. In recent times, most companies are raising capital towards tapping the growing demand and market for 3D (Taylor, 2014). The aim maintaining relevance drives them. The Mink is triggered by the level of profitability expected. The Mink is likely to be successful mainly because of the much hype it has received from the general population. People are always attracted by unique and new ways of doing things. People also like to experiment even with things that they do not necessarily need. Marketing strategies that have been adopted to market The Mink will also ensure this success. Through advertising, the innovation is receiving popularity with more people embracing the idea every day. It is evident that advertising influences the success of a business to a great extent. Through various exhibitions, awareness has been created regarding The Mink. Social media has also played a role in enhancing the popularity. The innovation is, therefore, likely to succeed. Furthermore, it is a common believe that women are impulse buyers; profits are likely to increase since they are the main users of the innovation. Nonetheless, the cosmetic industry is doing well in the business market (Restauri, 2014). Most women use

DNA Transformation in Bacteria

DNA Transformation in Bacteria 1.0 Introduction and Objectives The ability of bacteria to incorporate DNA from external sources is the primary reason for their survival and proliferation. Bacteria can take DNA from their surroundings or from other bacterial cells by cell wall-transfer. While an interesting phenomenon to examine for scientists, practically it is of great concern for the human race and a source of constant challenge for the Pharmaceutical Industry. The ability of bacteria to modify their genetic information has given rise to problems such as antibiotic resistance wherein bacteria become resistant to medications that were once effective in eliminating them. In this experiment, we examine the development of antibiotic resistance in bacteria. Circular DNA called plasmids are introduced in bacteria whose cells have been modified to promote uptake of plasmid DNA. This plasmid DNA will give rise to antibiotic resistance in the bacteria, which can be observed by allowing the bacteria to proliferate in an environment containing the antibi otic. Modification of genetic information in bacteria may be a source of concern, but that ability in the hands of humans has always been coveted. Genetic engineering is an increasingly popular research area given the breakthroughs made in recent years and the potential for commercial application. Various applications require large quantities of specific DNA sequences and this is where the bacterial ability to uptake DNA and reproduce it is beneficial. Introducing plasmids containing desired sequences into bacteria, allowing bacteria to reproduce and then isolating the required DNA is a common method used to obtain large quantities of particular DNA sequences. This aspect is also explored in this experiment. 1.1 Objectives The objectives of this experiment are to: a) Observe and examine the phenomenon of DNA Transformation. b) Observe the development of antibiotic resistance in bacteria through the process of gene transformation. c) Inculcate proper Sterile Technique for laboratory procedures involving bacterial strains. 2.0 Principles This section explores the underlying concept behind the experiment. Genetic Transformation is a process of horizontal gene transfer whereby DNA from the environment is taken up by a host cell. In this experiment bacterial cells are transformed. Escherichia Coli bacteria, which are generally non pathogenic are used in this experiment. The plasmids which constitute the external DNA contain a gene that makes the cell ampicillin resistant. Ampicillin is a bacteriostatic and will normally prevent the reproduction of E. Coli bacteria. This provides us with an easy way to test if gene transformation has occurred and to what extent by means of calculating the transformation efficiency. The introduction of genetic material within the bacterial cell is done by the process of electroporation. Electroporation involves applying an electrical voltage across the bacterial cells containing the plasmids. The ionic concentration of the DNA is kept low to prevent arcing. When the voltage is applied, holes open up in the walls of the bacteria. The plasmids can then enter the bacterial cells through these holes. Application of the voltage is done for a very short period of time. As soon as electric current stops flowing, the holes in the cell wall begin to close. A nutrient rich medium is then added to the bacterial cells, some of which will have transformed, to aid cell recovery. Incubation is then carried out, after which the cell suspension is diluted further and applied to agar plates containing the antibiotic. The cells are left to incubate for up to 24 hours and then the number of colonies determined. Calculating the transformation efficiency gives us a method to determine the extent to which the transformation occurred. 3.0 Methods and Materials 3.1 Materials The equipment and materials required for this experiment are outlined in this section. Equipment Required: A shaking incubator operating at 37ÃÅ'Ã…  C A non-shaking incubator An electroporator Materials Required: Cells treated for competency 2 agar plates with ampicillin with a concentration of 100 Â µg/ml pUC-19 plasmids 0.1 cm cuvettes Ice in an ice-box Deionised ultrapure water S.O.C. medium at room temperature 2 tubes with snap caps with a volume of 15 ml 3.2 Sterile Technique Sterile Technique is a must when handling pathogenic strains of bacteria. In this experiment, nonpathogenic bacterial strains are employed. However, using sterile technique is still good experimental procedure and promotes safety. Using sterile technique prevents errors in experimental results by preventing contamination from the surroundings. It also prevents contamination of the surrounding environment by the bacterial strain. Steps employed to prevent contamination included: Carrying out the experiment in an uncluttered area. Utilizing a fume hood to perform all procedures involving the bacteria. Washing hands both before as well as after the experiment Disposing off all bacterial waste in the appropriate container for bio-hazardous materials. 3.3 Procedure 3.3.1 Preparation for Electroporation The 0.1 cm cuvettes were cooled on ice. The electroporator was prepared based on prescribed settings. In order to bring the S.O.C. medium to room temperature, it was removed from the ice box. The cells and plasmids were allowed to thaw in the ice-box. Plates were heated at 37ÃÅ'Ã…  C to prepare for the incubation process. 3.3.2 Procedures I Â µl of pUC19 control DNA and 1 Â µl of ultrapure water were added to 2 separate microcentrifuge tubes with the aid of a pipette. The tube was then placed in the ice-box. 25 Â µl of competent cells were added to each of the microcentrifuge tubes. The contents of the tubes were gently mixed. Care was taken to avoid usage of the pipette for mixing. The tubes were then returned to the ice-box for 1 minute. The contents of each microcentrifuge tube were transferred to a cuvette using a pipette. It was ensured that the cells made contact with the cuvette walls and that no air-bubbles were present. This step was done rapidly to prevent heating up of the cells. The cuvettes were then electroporated. 250 Â µl of S.O.C. medium was added to the cells immediately after electroporation. Each of the two suspensions was transferred to a 15 ml tube. The shaking incubator was then set to 225rpm and used to incubate the cells for an hour to allow expression of the acquired antibiotic resistance. 10 Â µl of the transformed sample was then added to 90 Â µl of S.O.C. medium. The plates containing the ampicillin were then used. 20 Â µl of each of the two diluted samples from step 7 was added to a plate. Even spreading of the sample on the agar medium was ensured. Using the non-shaking incubator, the plates were incubated at 37 ÃÅ'Ã…  C for a day and the results recorded. 4.0 Results and Discussion 4.1 Results Answers to Questions (1) Schematic of observations of the agar plates: Figure 1: Results as Indicated by the Agar Plates (2) Count the colonies and calculate the transformation efficiency. Number of colonies observed = 13 Figure 2: Calculation of Transformation Efficiency Using the formula shown in figure 2, Transformation efficiency = 1.78 1010 transformants/Â µg plasmid DNA 4.2 Discussion Answers to Questions (1) Define the vocabulary used in this experiment: transformation, electroporation, host, plasmid, and competent. -Transformation Transformation is a process of horizontal gene transfer whereby DNA present in the environment of a cell is taken up by the cell. In this experiment the transformation involves the uptake of a plasmid containing a marker that results in ampicillin resistance by E. Coli bacteria through electroporation. -Electroporation Electroporation involves subjecting cells to an electric voltage to create holes in the cell wall. External material can then enter the cell through these holes. Natural processes then cause the hole to close and return the cell to its original state. -Host An organism that harbours a parasite is called a host. -Plasmid A plasmid is circular extra-chromosomal DNA. -Competent A competent cell is one which can internalise DNA present in its external environment. Competence can either be natural or artificial. (2) State why E. coli is used in many genetic engineering experiments. The popularity of Escherichia Coli for genetic experiments is due to various reasons. Firstly, most E. Coli strains are non-pathogenic and pose no harm to humans. Safety is a significant factor in the laboratory and E. Coli use is generally safe. Secondly, E. Coli grow easily and can be duplicated through metagenics. Thirdly, their genetic make-up is relatively simple and can be manipulated with ease. Fourthly they have been extensively studied and a lot is known about them. This makes it easier for researchers and they therefore prefer to use E. Coli for genetic engineering experiments. (3) Explain why competent cells, ampicillin, and S.O.C. medium were used for the transformation. Competent cells are necessary as transformation involves taking external genetic material into the cell. If cells are not competent this cannot happen and the experiment cannot be carried out successfully. Ampicillin is an antibiotic. Specifically, it is a bacteriostatic for E. Coli. It helps distinguish between bacteria that have taken up the plasmid and those that have not. This is because the plasmid contains a marker that causes ampicillin resistance. E. Coli cells do not naturally contain the genetic sequence that causes ampicillin resistance. Thus, ampicillin selection is possible to distinguish between transformed cells and untransformed cells. S.O.C. medium contains the nutrients required to help cells stabilise after electroporation. Electroporation introduces holes into the cell wall of the cell and therefore causes destabilisation of the cell. S.O.C medium contains yeast extract and other nutrient sources that help the cell recover. Once the cell has recovered and if the plasmid has entered the cell during electroporation, the cell will multiply and give rise to a colony during the incubation period. (4) Explain the purpose of the controls in this experiment. The control in this experiment constitutes bacteria without the plasmid that inculcates antibiotic resistance. Without this extra piece of genetic information to enable the bacteria to mount defences against the attack of the antibiotic, ampicillin is this case, the bacterial cells will be unable to multiply in a medium that contains the antibiotic. The cells that were treated such that they could incorporate the plasmid DNA into their genetic make-up will be able to multiply in a medium where ampicillin is present as long as there are enough nutrients available for growth. Thus, the control helps us show that the DNA plasmid was indeed taken up and incorporated into their genetic make-up by the bacteria. The only way for E. coli to have survived with ampicillin present is if they had taken up the plasmid and transmitted it to all generations when they reproduced after uptake of the plasmid. Hence, the control serves to confirm uptake of the plasmid as well as its transmission to fol lowing generations by comparing it to cells in the control that did not have the extra DNA. (5) Explain how the colony growth relates to gene transformation. A colony of bacteria stems from the binary fission of one single bacterial cell. When bacteria reproduce vertical genetic transfer occurs whereby the offspring has the exact copy of the genetic material of the parent. In this experiment, bacteria are introduced into a medium containing the antibiotic ampicillin. E. Coli bacteria with their original genetic make-up will be unable to reproduce due to the presence of the antibiotic as they do not have the means necessary to resist antibiotic attack. This is what is expected in the control sample as ampicillin is a bacteriostatic.. The positive sample on the other hand has bacteria which have undergone horizontal gene transfer by transformation. The plasmid DNA that was used for the transformation process contains genetic code that results in E.Coli developing ampicillin resistance. Thus, bacteria that can incorporate this plasmid and pass it on to their offspring by vertical gene transfer can grow in the environment. This is how colony growth relates to gene transformation. (6) Describe how ionic strength of DNA solution affects electroporation. The ionic strength of DNA solution comes into play due to the electroporation stage where holes are created in the bacterial cell wall to allow uptake of the plasmid by transmission of an electric voltage. For this step, the ionic strength of the solution must be low. If the ionic strength is high, arcing will occur. Arcing is visible during the experiment by sparks and a sound like a micro-scale thunderclap. It can cause cell death as well as equipment damage. Thus, for the experiment to be carried out successfully and to safeguard the apparatus, the DNA solution must be of low ionic strength. (7) If your transformation efficiency is lower than 1 109 cfu/ÃŽ ¼g, conjecture and explain potential reasons for the low efficiency. The transformation efficiency is greater than the benchmark stated above. This corresponds to good transformation efficiency and indicates a successful transformation process. However, the close clustering of the colonies makes it possible that some of the colonies are satellite colonies rather than transformed colonies. The experiment could be repeated with a higher concentration of ampicillin to obtain more reliable results. (8) Discuss current and potential applications of gene transformation techniques in biotechnology. Gene transformation techniques play a crucial role in biotechnology. This is because gene transformation provides a method to produce copies of desired DNA sequences. This is especially useful in the pharmaceutical industry to develop medications that are target specific. Also, this could potentially lead the way to genetic engineering, where defects to the genetic code could be repaired and desired traits inserted through addition of the corresponding DNA sequences. Gene replacement therapy could prove to be the cure for nearly all diseases that take human lives contemporarily. In the future gene transformation could be used to engineer human beings and other animals and plants according to desired specifications. Genetic transformation is also used in the development of pest-resistant crops, which could potentially increase the productivity of the land. This could be key to feed the ever-growing population as the quantity of agricultural land decreases. Understanding the evolution of drug resistance could help us devise ways of preventing drug resistance as well as developing drugs that can overcome resistance. In this arena gene transformation plays an important role horizontal genetic transfer is a natural process in bacteria. 4.3 Sources of Error and Suggestions for Improvement There are a few sources of error that could result in incorrect conclusion being drawn from experimental results. (i) The number of colonies seen need not correspond to the bacteria that transformed. This could be due to the growth of satellite colonies. Large bacterial colonies will secrete beta lactamase, which is what causes ampicillin resistance. Thus, the area around the colony will contain this secretion and be ampicillin-free. A satellite colony could grow in this area from untransformed cells. To avoid this problem, the incubation period should strictly be restricted to 24 hours. Satellite colonies emerge after a delay. By ensuring that results arr recorded promptly, the interference in results brought about by satellite colonies can be minimised. Another method is to use a higher concentration of ampicillin. More time will be required to create a antibiotic-free zone around a colony if the concentration of antibiotic is high. (ii) Identifying the number of colonies can be difficult, especially if the size of the colony is miniscule. This could result in an incorrect calculation of transformation efficiency. In order to increase accuracy of results, a different selection marker can be used. Some selection markers have properties that can be distinguished by shining UV light and other such techniques which result in a high contrast. Using these markers may result in higher reliability of results. (iii) Distinguishing between colonies can be difficult if they grow close to one another and appear to be one large colony. Also, closer colonies would also result in a higher chance of there being satellite colonies. To minimise this problem, crowding on the plate must be minimised. For that, a higher concentration of ampicillin could be used, carbenicillin selection could be used instead of ampicillin selection (although expensive) or the nutrient dilution could be adjusted such that it discourages very rapid proliferation. 5.0 Conclusions The objectives of this experiment were to explore the phenomenon of gene transformation and the development of antibiotic resistance in bacteria as well as to inculcate the practice of sterile technique for handling bacteria. Gene transformation was observed with the development of ampicillin resistance in transformed Escherichia Coli bacteria. The bacteria not exposed to the plasmids containing the genes for antibiotic resistance did not grow in an environment containing the antibiotic while the transformed bacteria formed colonies in the same environment. A calculation of transformation efficiency returned a value of 1.78 1010 transformants/Â µg of plasmid DNA, which is greater than the threshold of 109, indicative of a successful experiment. However, the possibility of some of the 13 colonies of bacteria being satellite colonies as opposed to transformed colonies reduces the reliability of the results. Methods to increase reliability of results were therefore suggested. References 1. Port, Tami. (2008, June 14). Bacteria Horizontal Gene Transfer. suite101.com. Retrieved 3rd April, 2010 from http://bacteriology.suite101.com/article.cfm/bacteria_horizontal_gene_transfer 2. Metzenberg, Stan. (2002). Bacterial Plasmids. California State University Northridge Department of Biology. Retrieved 4th April, 2010 from http://escience.ws/b572/L2/L2.htm

Significant Event

One of these events stands out in particular to me and had he greatest impact in my life. Physically disciplining a child has a dramatic impact on both the child as well as the parent. As a child I remember that for any little misbehaver resulted in a physical punishment as well as getting yelled at, as time went by my mind was set that I already knew when the â€Å"beating† was going to come. My family continue to be very big on respect and discipline, but at the time little did they know that by hitting us they weren't just punishing us for a little while, they were in fact pushing us away and causing frustration, at times even hatred.I also researched that in result of physically harming children increases their risk of mental illness, in which resulted when I came upon this article which states that â€Å"It's pretty well established that physically harming children has a negative impact on mental health, but this is showing the same effect even when you look at milder fo rms of physical force. This is saying that physical punishment should not be used on children of any age. † Each perspective has its strengths and weaknesses, which brings difference to our understanding of the human behavior.In my situation am going to go with the behavioral perspective, as well as the psychodrama perspective. By looking at the physical punishment from a behaviorism perspective, conditioning by pain requires that the consequence always occurs immediately after every incident. The psychodrama perspective states that in childhood certain incidents may occur that produce behaviors in their adulthood. Many different conflicts throughout childhood development shape overall personality. Observational learning refers to learning that occurs as a function of observing, and placating behavior observed in which is particular during childhood.I later figured out that the way my mother and aunts were physically punishing me, resulted in the fact that they learned from my grandmother, they completely mimicked the way my grandmother punished them. As I grew older I began to express myself to them in a matter that I felt curious I began to question why would they always physically harm my cousins as well as myself, their excuse still remains the exact same in which they say † That it is simply something they grew up seeing on a daily basis†. SST memories are not always accurate which can result in a blur, but do believe that it depends on how intense was the situation. There are many memories do vividly remember from my childhood but they are also plenty which don't necessarily remember everything, at times will looking at certain photos and remember exactly what I did that particular day, so I do think that it all just depends on the importance of the situation. Although do in fact accurately remember many different situations in which was hit, many of which I now think back ND know that could have been easily solved with just a simple co nversation.

White King, Red Rubber, Black Death

White King, Red Rubber, Black Death â€Å"White King, Red Rubber, Black Death,† illustrated how King Leopold II of Belgium acquired the Congo as a free state and exploited it by reign of terror. King Leopold II took over leadership of Belgium from his father, hoping to gain power and wealth, as well as assuming control of overseas territories like most other European nations of the time did. Leopold created, through political lobbying and military force, the Belgian Free State.The Belgian Free State gave Leopold power over the African territory of the Congo, which he soon exploited for its large supplies of ivory and rubber. Leopold and his soldiers used the Congolese natives as forced labor, and those who refused to work for the Belgians or who violated their newly established laws were punished by mutilation, torture, or death. Nearly ten million people were either slaughtered or worked to death in the Congo under Leopold's rule.In the end, Leopold's reign of terror in the C ongo became a scandal during the last years of his rule, and he destroyed most of the documents pertaining to the Belgian Free State prior to his death. Before this History class, I would have never had known about the documentary â€Å"White King, Red Rubber, Black Death† and about the many issues that were present in the Congo of Africa in the past. This documentary had left me speechless and astonished in the end. It had truly opened my eyes to the many tragedies that the Africans of the Congo endured under the reign of King Leopold II.Furthermore, I wondered, why throughout the entire reign of King Leopold II didn’t anyone even try bringing him down and making him pay for his cruel punishments depicted upon the Congo popularity? It amazes me that, during the thirty years that Leopold committed acts of violence and cruelty amongst the Congo, Leopold was still worshiped after by the Belgium’s as the man who colonized the Congo and made the Belgium city wealthy . This is a man who depicted the ignorance and greediness of Belgium.In my eyes, everything that Belgium has today, is out of fraud and lies because of the selfish acts Leopold committed. All of this cruelty, because of the exportation of rubber? I couldn’t believe it with my own eyes and ears, the amount of damage Leopold had caused amongst the Congo and its people just because of his obsession with power and wealth. Yet, if this was an issue that was being presented in today’s society of a specific area many would jump to fight and defend this area.King Leopold conducted many extreme measures in the past for no reason, in my opinion. If I was alive during this period and was one of the many people of the Congo who were enduring this cruelty and pain, I would stand up for what I believe in, save my people, and make a change. I do believe that if such person in today’s society, conducted these acts of punishment they would immediately get the death penalty becau se of the well improved justice system we have today, but why not back then?